Molecular identification of onion yellow dwarf virus in Allium fistulosum and Allium schoenoprasum in Indonesia
Penulis/Author
Dr. Adyatma Irawan Santosa, S.P., M.Sc. (1); Dyana Rahma Ningrum (2); Siska Nur Siti Yuliandini (3); Alifia Nur Ayusma (4); Muh Amat Nasir, S.P., M.Sc. (5); Dr. Aprilia Sufi Subiastuti, S.Si. (6)
Tanggal/Date
5 2024
Kata Kunci/Keyword
Abstrak/Abstract
Virus infection in four edible Allium spp.: Welsh onion (Allium fistulosum), onion (Allium cepa), fresh chive (Allium
schoenoprasum), and garlic chive (Allium tuberosum) cultivated in Indonesia had never been studied. Samples were
obtained during field surveys in June – October 2023 at different production centers in Java Island, Indonesia, and then
molecularly tested using a universal primer pair to amplify ± 700 bp of the 3′-end region of potyviruses. Six Welsh onion
and four fresh chive samples were tested positive for Onion yellow dwarf virus (OYDV). Phylogenetic tree clustered the
sequenced four Indonesian Welsh onion isolates (GenBank acc. no. OR772075 – 78) in a group together with other Welsh
onion isolates from South Korea and Japan. Meanwhile, the four fresh chive isolates (OR772079 – 82) that were obtained
were positioned in a separate group together with predominantly onion isolates from Europe and America. No significant
recombination signal was detected on the compared isolates using Recombination Detection Program v5.30. Percentage
identity analysis further determined the divergence of Welsh onion isolates to isolates from other hosts.
