Karya
Judul/Title Coat Protein 1 Gene of Indonesian Isolates of Rice Tungro Spherical Virus showed High Divergence with those from other South and South East Asian Regions
Penulis/Author Raden Heru Praptana (1); Dr. Adyatma Irawan Santosa, S.P., M.Sc. (2) ; Rahmini (3); Wasis Senoaji (4); Nur Rosida (5); Danarsi Diptaningsari (6); Affandi (7); Riska (8); Prof. Dr. Ir. Y. Andi Trisyono, M.Sc. (9); Dr. Ir. Sedyo Hartono, M.P. (10)
Tanggal/Date 4 2023
Kata Kunci/Keyword
Abstrak/Abstract Background: Tungro, an important viral disease in rice, is a co-infection of rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV). Severe symptoms were reported in rice fields on two major islands in Indonesia: Jawa (Magelang District) and Sulawesi (Sidrap District). Objective: Sequences of global isolates currently registered in NCBI GenBank were examined with two new Indonesian isolates to understand the phylogeny of RTSV based on different genome regions as well as to spot the gap in the molecular data of Indonesian isolates. Methods: Transmission test of RTSV using the vector green leaf hopper was performed, and then the complete sequences of coat protein 1 (CP1) gene of Magelang and Sindrap districts were molecularly studied using MEGA X software and Recombination Detection Program. Results: The obtained complete sequence of coat protein 1 (CP1) gene of Magelang-7 (OQ426495) and Sindrap-9 (OQ426496) isolates were aligned with 39 isolates in NCBI GenBank. Sequences of other isolates in GenBank were also retrieved and then aligned according to six individual Open Reading Frames (ORFs) and ‘Full ORF’. Recombination analysis detected significant signals in the genomes of one partially and four fully sequenced isolates. Phylogenetic study also confirmed the frequent recombination among RTSV isolates clustered in two geographically separated groups: South East Asia and South Asia. Conclusion: Although the available data is still rudimentary, this study demonstrated that the CP1 gene of 21 isolates from different regions of Indonesia was highly identical among themself but divergent with isolates from other countries. Therefore, deep molecular comprehension on Indonesian isolates is necessary to manage the recurrent Tungro spread in the country.
Rumpun Ilmu Ilmu Hama dan Penyakit Tanaman
Bahasa Asli/Original Language English
Level Internasional
Status
Dokumen Karya
No Judul Tipe Dokumen Aksi
1Praptana etal2023-CP1 gene of Indonesian isolates of RTSV showed high divergence.pdf[PAK] Full Dokumen